Journal: Genome Biology

Article Title: SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs

doi: 10.1186/s13059-021-02497-7

Figure Lengend Snippet: SRCP accurately annotates circRNAs. A Venn diagram of the circRNAs found by the circRNA-identification pipelines in analysis of the total RNA library from the GSE55872 dataset. For this and further analysis, we utilized only circRNAs which were found in the mock samples. B RNaseR/mock ratio distribution in Drosophila melanogaster . The data in orange represent the circular junctions and that in violet the linear junctions. For the linear junctions, we utilized the SRCP output of the mRNAs produced from the genes hosting the potential circRNAs. C The number of circRNAs identified as “true” positives as a function of the cutoff for circRNAs identified by 1, 2, 3, 4, or 5 of the pipelines used. The dotted lines indicate three potential threshold/cutoffs (0.85, 0.9, or 0.95 respectively). The cutoff is defined as the fraction of linear mRNAs that would have some resistance to RNaseR. D Number of true and false circRNAs that have been identified from 1, 2, 3, 4, or 5 pipelines for different cutoffs in ( C ). E Boxplots showing the distribution of expression (top) and the RNaseR/mock ratio (bottom) of the true and false circRNA that are identified either by 3 (right), 4 (middle), or 5 (left) pipelines. F Percent of true and false positives identified by SCRP and each individual circRNA-identification pipeline

Article Snippet: Human Adult Normal Brain Total RNA (R1234035-50), Human Adult Normal Cerebellum Total RNA (R1234039-50), Rat Brain Total RNA (R1434035-50), Monkey (Rh) Brain (R1534035-50) Total RNA, and Human Blood Total RNA (custom made) were obtained by BioChain.

Techniques: Produced, Expressing